![]() The tests which performed the best were the Gull Premier EIA (sensitivity, 86.7% specificity 97.6%), and the Kalon Biological (sensitivity, 92.3% specificity, 97.7%) tests. Test sensitivities from 86% to 100%, while the specificity ranged from 47-99%. The discordant assays and the three best assays were compared to the Western blot assay from the University of Washington. Test samples were first compared to monoclonal antibody (MAb) enzyme immunoassays (EIA) from the Central Public Health Lab, London, UK. Performance of Commercially Available Enzyme Immunoassays for Detection of Antibodies against Herpes Simplex Virus Type 2 in African Populations. One review compared thirteen available commercial immunoassays for HSV-2 4 van Dyck, et al., 2004. DISCUSSION: Several reports have analyzed available commercial assays in the past. Sexually Transmitted Diseases Treatment Guidelines. False-positive results can bring emotional stress to the patient, and for this reason, a second test is often requested for a confirmatory result. The inaccuracy of Certain Commercial Enzyme Immunoassays in Diagnosing Genital Infections with Herpes Simplex Virus Types 1 and 2. While this FDA approved assay is considered to be very accurate, a second confirmatory assay is often preferred as most patients are unaware of their genital Herpes status 2 Ashley-Morrow, Rhoda, and David Friedrich, 2003. Of these, the University of Washington Western blot assay (UW-WB) is considered the “gold standard” for assays to detect Herpes virus antibodies in patient sera 1 University of Washington Western blot. INTRODUCTION: There are several commercial immunoassays for HSV on the market (Western blot, Dot blot or standard ELISA assays). By David Kilpatrick, PhD and Abbas Vafai, PhD ![]()
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